Transposon Insertional Mutagenesis in Rice,
نویسندگان
چکیده
منابع مشابه
Transposon insertional mutagenesis in rice.
Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands (R.G., A.P); Institute of Molecular Plant Sciences Leiden University, The Netherlands (P.B.F.O., J.H.C.H); Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Montpellier, France (C.S., E.G.); John Innes Centre, Norwich, United Kingdom (A.K., P.C.); University of Milan, Italy (L.C.); ...
متن کاملT-DNA insertional mutagenesis for functional genomics in rice.
We have produced 22 090 primary transgenic rice plants that carry a T-DNA insertion, which has resulted in 18 358 fertile lines. Genomic DNA gel-blot and PCR analyses have shown that approximately 65% of the population contains more than one copy of the inserted T-DNA. Hygromycin resistance tests revealed that transgenic plants contain an average of 1.4 loci of T-DNA inserts. Therefore, it can ...
متن کاملT-DNA insertional mutagenesis for activation tagging in rice.
We have developed a new T-DNA vector, pGA2715, which can be used for promoter trapping and activation tagging of rice (Oryza sativa) genes. The binary vector contains the promoterless beta-glucuronidase (GUS) reporter gene next to the right border. In addition, the multimerized transcriptional enhancers from the cauliflower mosaic virus 35S promoter are located next to the left border. A total ...
متن کاملTransposon insertional mutagenesis in mice identifies human breast cancer susceptibility genes and signatures for stratification.
Robust prognostic gene signatures and therapeutic targets are difficult to derive from expression profiling because of the significant heterogeneity within breast cancer (BC) subtypes. Here, we performed forward genetic screening in mice using Sleeping Beauty transposon mutagenesis to identify candidate BC driver genes in an unbiased manner, using a stabilized N-terminal truncated β-catenin gen...
متن کاملTransposon-mediated insertional mutagenesis revealed the functions of animal cellulose synthase in the ascidian Ciona intestinalis.
Tunicates are the only animals that perform cellulose biosynthesis. The tunicate gene for cellulose synthase, Ci-CesA, was likely acquired by horizontal transfer from bacteria and was a key innovation in the evolution of tunicates. Transposon-based mutagenesis in an ascidian, Ciona intestinalis, has generated a mutant, swimming juvenile (sj). Ci-CesA is the gene responsible for the sj mutant, i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 2001
ISSN: 1532-2548,0032-0889
DOI: 10.1104/pp.125.3.1175